Storing Sclerotinia
Filter paper
Inoculate a small agar plug from an actively growing culture onto a Potato Dextrose Agar plate and place sterile Whatman #1 filter paper pieces around the inoculum plug.
Allow hyphae to colonize the filter paper. Culture is mature when sclerotia are dry.
Remove colonized filter paper with forceps, place in a sterile key envelope and dry in a desiccator for about a week.
Store envelopes at –20°C in an airtight plastic container that has Drierite desiccant in the bottom.
Dry Sclerotia
Shake mature sclerotia from a PDA culture onto a small petriplate and let air dry. When sclerotia are completely dry (rattle when shaked), place in a vial and store at +4°C in a container containing Drierite desiccant.
Mycelia in DMSO
From an actively growing culture on PDA, cut discs with a cork borer and place in a vial containing 15 % DMSO in sterile water. Store at –80°C. Corkborer#2 (5 mm) plugs fit into cryovials, #3 (8mm) is suitable for 1.5 mL and 2 mL tubes.
Updated 28 May 2007 by Jeffrey Rollins.
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