Producing Sclerotia on Plates
- Chop one baking potato (200 g) and autoclave for 30 min in a beaker in 600 mL of water.
- Blend or mash when cooled and pour puree into a 1 L flask, adjusting volume to 600 mL.
- Add 9 g agar, mix and reautoclave. Pour 6-7 plates (150×15).
- Inoculate in the middle of the plate, with one dry sclerotium. Incubate at room temperature.
- At three weeks, or when sclerotia are mature and before they start growing again, place plates, open, in a hood to dry.
- When agar is bone-dry (2-3 days), pick or shake sclerotia off. DO NOT WASH. Dry in a hood overnight and store at +4°C.
Updated 28 May 2007 by Jeffrey Rollins.
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